Cat#:Y04701
Species:Human
Size:10μg/50μg/1mg
Purity:≥95%
Endotoxin: ≤10 EU/mg
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Y04701 Recombinant Human VEGF165
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Application Details Cat. No.
Y04701
Purity
≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)
Endotoxin
≤10 EU/mg by the LAL method
Bioactivity
Measured in a cell proliferation assay using HUVEC human umbilicveinendothelial cells. The ED50 for this effect is 0.2-5 ng/mL
Accession
P15692
Alternative Names
Vascular Endothelial Growth Factor Isoform 165; VEGF165
Species
Human
Expression System
CHO
Target Protein Sequence
APMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQENPCGPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR
Mol. Weight
19.1kDa
Expression Region
Ala27-Arg191
Tag Info
Tag free
Formulation
Lyophilized
Buffer
PBS, 5% mannitol and 0.01% Tween 80, pH 7.4
Size
10μg/vail 50μg/vail 1mg/vail
Lead Time
about 60 working days
Reconstitution
It is recommended to redissolve in sterile deionized water.
Shipping
The product is shipped with blue ice
Storage & Stability
36 months at 2°C to 8°C in lyophilized state
6 months at -20°C to -80°C under sterile conditions after reconstitution
7-10 days at 2°C to 8°C under sterile conditions after reconstitution
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
SDS-PAGE
Recombinant Human VEGF165 (Cat. No. Y04701) SDS-PAGE under reducing (R) & Non-reducing conditions. The gel was stained with Y04701 SDS-PAGE.
Bioactivity
Recombinant Human VEGF165 (Cat. No. Y04701) stimulates proliferation of HUVEC human umbilicvein endothelial cells. The ED50 for this effect is 0.2-10 ng/mL. Y04701 was higher than the other competing product
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