Cat#:Y04401
Name:Y04401 Recombinant Human TNFα
Species:Human
Specification:10μg/50μg/1mg
Purity:≥95%
Endotoxin: ≤10 EU/mg
Expression System:CHO
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Y04401 Recombinant Human TNFα
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Product Details Cat. No.
Y04401
Purity
≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)
Endotoxin
≤10 EU/mg by the LAL method
Bioactivity
Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 5-60 pg/mL.
Accession
P01375
Alternative Names
umor Necrosis Factor; Cachectin; TNF-Alpha; Tumor Necrosis Factor Ligand Superfamily Member 2; TNF-a; TNF; TNFA; TNFSF2
Species
Human
Expression System
CHO
Target Protein Sequence
VRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL
Mol. Weight
17.4 kDa
Expression Region
Val77-Leu233
Tag Info
Tag free
Formulation
Lyophilized
Buffer
PBS,5% mannitol and 0.01% Tween 80, pH7.4
Size
10μg/vail 50μg/vail 1mg/vail
Lead Time
Products are in stock,but custom products may require further consultation
Reconstitution
It is recommended to redissolve in sterile deionized water.
Shipping
The product is shipped with blue ice
Storage & Stability
36 months at 2°C to 8°C in lyophilized state
6 months at -20°C to -80°C under sterile conditions after reconstitution
7-10 days at 2°C to 8°C under sterile conditions after reconstitution
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
SDS-PAGE
Recombinant Human TNFα (Cat. No. Y04401) SDS-PAGE under reducing (R) & Non-reducing conditions. The gel was stained with Y04401 SDS-PAGE.
Bioactivity
Recombinant Human TNFα (Cat. No. Y04401) measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 5-60 pg/mL. TheBioactivity of Y04401 was equivalent to other competing products.
Bioactivity
Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D.
SEC-HPLC
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