Cat#:Y04701
Species:Human
Size:10μg/50μg/1mg
Purity:≥95%
Endotoxin: ≤10 EU/mg
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Y04701 Recombinant Human VEGF165
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Application Details Cat. No.
Y04701
Purity
≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)
Endotoxin
≤10 EU/mg by the LAL method
Bioactivity
Measured in a cell proliferation assay using HUVEC human umbilicveinendothelial cells. The ED50 for this effect is 0.2-5 ng/mL
Accession
P15692
Alternative Names
Vascular Endothelial Growth Factor Isoform 165; VEGF165
Species
Human
Expression System
CHO
Target Protein Sequence
APMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQENPCGPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR
Mol. Weight
19.1kDa
Expression Region
Ala27-Arg191
Tag Info
Tag free
Form
Lyophilized
Buffer
PBS, 5% mannitol and 0.01% Tween 80, pH 7.4
Size
10μg/vail 50μg/vail 1mg/vail
Lead Time
Products are in stock, but custom products may require further consultation.
Reconstitution
It is recommended to redissolve in sterile deionized water.
Shipping
The product is shipped with blue ice
Storage & Stability
36 months at 2°C to 8°C in lyophilized state
6 months at -20°C to -80°C under sterile conditions after reconstitution
7-10 days at 2°C to 8°C under sterile conditions after reconstitution
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
SDS-PAGE
Recombinant Human VEGF165 (Cat. No. Y04701) SDS-PAGE under reducing (R) & Non-reducing conditions. The gel was stained with Y04701 SDS-PAGE.
Bioactivity
Recombinant Human VEGF165 (Cat. No. Y04701) stimulates proliferation of HUVEC human umbilicvein endothelial cells. The ED50 for this effect is 0.2-10 ng/mL. Y04701 was higher than the other competing product
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