Cat#:Y00801
Species:Human
Size:10μg/50μg/1mg
Purity:≥95%
Endotoxin: ≤10 EU/mg
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Y00801 Recombinant Human EGF
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Application Details Cat. No.
Y00801
Purity
≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)
Endotoxin
≤10 EU/mg by the LAL method
Bioactivity
Measured in a cell proliferation assay using NIH3T3 mouse embryonic fibroblast Cells. The ED50 for this effect ≤0.3 ng/mL.
Accession
P01133
Alternative Names
Pro-Epidermal Growth Factor; EGF; Epidermal Growth Factor; Urogastrone
Species
Human
Expression System
CHO
Target Protein Sequence
NSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELR
Mol. Weight
6.2 kDa
Expression Region
Asn971-Arg1023
Tag Info
Tag free
Form
Lyophilized
Buffer
PBS,5% mannitol and 0.01% Tween 80, pH7.4
Size
10μg/vail 50μg/vail 1mg/vail
Lead Time
Products are in stock,but custom products may require further consultation
Reconstitution
It is recommended to redissolve in sterile deionized water.
Shipping
The product is shipped with blue ice
Storage & Stability
36 months at 2°C to 8°C in lyophilized state
6 months at -20°C to -80°C under sterile conditions after reconstitution
7-10 days at 2°C to 8°C under sterile conditions after reconstitution
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
SDS-PAGE
Recombinant Human EGF (Cat. No. Y00801) SDS-PAGE under reducing (R) & Non-reducing condition. The gel was stained with Y00801 SDS-PAGE
Bioactivity
Recombinant Human EGF (Cat. No. Y00801) stimulates proliferation of NIH3T3 cells. The ED50 for this effect ≤0.3 ng/mL. The acivity of Y00801 was equivalent to other competing products.
Bioactivity
Highly consistent bioactivity between different production batches of Recombinant Human Transferrin (Cat. No. Y00801).
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