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Y01311 Recombinant Human Fibronectin

Y01311 Recombinant Human Fibronectin

Cat#:Y01311

Species:Human

Size:10μg/50μg/1mg

Purity:≥95%

Endotoxin: ≤10 EU/mg

Application Details
Cat. No.

Y01311

Purity

≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)

Endotoxin

≤10 EU/mg by the LAL method

Bioactivity

Measured by the ability of the immobilized protein to  support the adhesion of NIH3T3 mouse embryonic fibroblast cells. The ED50 

for this effect is 35.0-420 ng/mL.

Accession

P02751

Alternative Names

FN; Cold-insoluble globulin; CIG; FN; Fibronectin 1

Species

Human

Expression System

E.coli

Target Protein Sequence

PTDLRFTNIGPDTMRVTWAPPPSIDLTNFLVRYSPVKNEEDVAELSISPSDNAVVLTNLLPGTEYVVSVSSVYEQHESTPLRGRQKTGLDSPTGIDFSDITANSFTVHWIAPRATITGYRIRHHPEHFSGRPREDRVPHSRNSITLTNLTPGTEYVVSIVALNGREESPLLIGQQSTVSDVPRDLEVVAATPTSLLISWDAPAVTVRYYRITYGETGGNSPVQEFTVPGSKSTATISGLKPGVDYTITVYAVTGRGDSPASSKPISINYRTEIDKPSMAIPAPTDLKFTQVTPTSLSAQWTPPNVQLTGYRVRVTPKEKTGPMKEINLAPDSSSVVVSGLMVATKYEVSVYALKDTLTSRPAQGVVTTLENVSPPRRARVTDATETTITISWRTKTETITGFQVDAVPANGQTPIQRTIKPDVRSYTITGLQPGTDYKIYLYTLNDNARSSPVVIDASTAIDAPSNLRFLATTPNSLLVSWQPPRARITGYIIKYEKPGSPPREVVPRPRPGVTEATITGLEPGTEYTIYVIALKNNQKSEPLIGRKKTDELPQLVTLPHPNLHGPEILDVPST

Mol. Weight

62.6 kDa

Expression Region

Pro1270-Ser1546 & Ala1721-Thr2016

Tag Info

Tag free

Formulation

Lyophilized

Buffer

PBS,5% mannitol and 0.01% Tween 80, pH7.4

Size

10μg/vail 50μg/vail 1mg/vail

Lead Time

Products are in stock,but custom products may require further consultation  

Reconstitution

It is recommended to redissolve in sterile deionized water.

Shipping

The product is shipped with blue ice

Storage & Stability

36 months at 2°C to 8°C in lyophilized state

6 months at -20°C to -80°C under sterile conditions after reconstitution

7-10 days at 2°C to 8°C under sterile conditions after reconstitution

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Data

SDS-PAGE

Y01311 Recombinant Human Fibronectin.jpg

Recombinant Human Fibronectin (Cat. No. Y01311) SDS-PAGE 

under reducing (R) & Non-reducing conditions. The gel was 

stained with Y01311 SDS-PAGE.


Bioactivity

Y01311.png

Recombinant Human Fibronectin (Cat. No. Y01311) stimulates 

adhesion of NIH3T3 cells. The ED50 for this effect is 

35.0-420 ng/mL. The bioactivity of Y01301 was higher than 

other competing products.     

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