Cat#:Y03501
Species:Human
Size:10μg/50μg/1mg
Purity:≥95%
Endotoxin: ≤10 EU/mg
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Y03501 Recombinant Human R-Spondin1
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Application Details Cat. No.
Y03501
Purity
≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)
Endotoxin
≤10 EU/mg by the LAL method
Bioactivity
Measured by its ability to induce Topflash reporter bioactivity in CHO cells in the presence of 50ng/ml wnt3a. The expected ED50 is <1.0 μg/mL
Accssion
Q2MKA7
Alternative Names
RSPO1; R-spondin1; RP11-566C13.1; CRISTIN3; FLJ40906; RSPO Rspo1; R-spondin; Rspondin; RP23-325M14.2; Roof
Species
Human
Expression System
CHO
Target Protein Sequence
SRGIKGKRQRRISAEGSQACAKGCELCSEVNGCLKCSPKLFILLERNDIRQVGVCLPSCPPGYFDARNPDMNKCIKCKIEHCEACFSHNFCTKCKEGLYLHKGRCYPACPEGSSAANGTMECSSPAQCEMSEWSPWGPCSKKQQLCGFRRGSEERTRRVLHAPVGDHAACSDTKETRRCTVRRVPCPEGQKRRKGGQGRRENANRNLARKESKEAGAGSRRRKGQQQQQQQGTVGPLTSAGPA
Mol. Weight
26.7 kDa
Expression Region
Ser21-Ala263
Tag Info
Tag free
Formulation
Lyophilized
Buffer
PBS,5% mannitol and 0.01% Tween 80, pH7.4
Size
10μg/vail 50μg/vail 1mg/vail
Lead Time
Products are in stock,but custom products may require further consultation
Reconstitution
It is recommended to redissolve in sterile deionized water.
Shipping
The product is shipped with blue ice
Storage & Stability
36 months at 2°C to 8°C in lyophilized state
6 months at -20°C to -80°C under sterile conditions after reconstitution
7-10 days at 2°C to 8°C under sterile conditions after reconstitution
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
SDS-PAGE
Recombinant Human R-Spondin1 (Cat. No. Y03501) SDS-PAGE
under reducing (R) & Non-reducing conditions. The gel was
stained with Y03501 SDS-PAGE.
Bioactivity
Measured by its ability to induce Topflash reporterBioactivity in
CHO cells in the presence of 50ng/ml wnt3a. The expected
ED50 is＜1.0 μg/ml. The bioactivity of Recombinant Human
R-Spondin-1(cat no Y03501) was higher than other
competing products.
Bioactivity
Highly consistent bioactivity between different production
batches of Recombinant Human R-Spondin1
(Cat. No. Y03501).
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