Y00341 Recombinant Fish bFGF Product Brochure

Cat#:Y00341

Name:Y00341 Recombinant Fish bFGF

Species:Fish

Specification:50μg

Purity:≥95%

Endotoxin: ≤200EU/mg

Expression System:E.coli

Online Consultation

Related applications

Product Details
Data
Related Products

Product Details

Cat. No.

Y00341

Purity

≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)

Endotoxin

≤200 EU/mg by the LAL method

Bioactivity

Measured in a cell proliferation assay using NIH3T3 mouse embryonic fibroblast cells. The ED₅₀ for this effect is ≤2.0 ng/mL

Accession

XP_030252529.1

Alternative Names

Fibroblast Growth Factor 2; FGF-2; Basic Fibroblast Growth Factor; bFGF; Heparin-Binding Growth Factor 2; HBGF-2; FGF2; FGFB

Species

Fish

Expression System

E. coli

Target Protein Sequence

MATGEITTLP STPEDGGSGG FPPGSFKEPK RLYCKNGGFF LRIKSDGGVD GIREKNDPHI KLQLQATSVG EVVIKGVCAN RYLAMNRDGR LFGARRATDE CHFLERLESN NYNTYRSRKY PNMYVALKRT GQYKSGSKTG PGQKAILFLP

Mol. Weight

17.1 kDa

Expression Region

Met1-Cys155

Tag Info

Tag free

Formulation

Lyophilized

Buffer

10 mM PB, 250 mM NaCl, 5% mannitol and 0.01% Tween 80, pH7.4

Size

50μg/vail

Lead Time

Products are in stock, but custom products may require further consultation.

Reconstitution

It is recommended to redissolve in sterile deionized water.

Shipping

The product is shipped with blue ice.

Storage & Stability

36 months at 2°C to 8°C in lyophilized state

6 months at -20°C to -80°C under sterile conditions after reconstitution

7-10 days at 2°C to 8°C under sterile conditions after reconstitution

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Data

Bioactivity

Recombinant Fish bFGF (Cat. No. Y00341) stimulates proliferation of NIH3T3 cells. The ED₅₀ for this effect is >2.0 ng/mL. The bioactivity of recombinant Fish bFGF (Cat. No. Y00341) was higher than the other competing product.