Cat#:Y01311
Name:Y01311 Recombinant Human Fibronectin
Species:Human
Specification:10μg/50μg/1mg
Purity:≥95%
Endotoxin: ≤10 EU/mg
Expression System:E.coli
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Y01311 Recombinant Human Fibronectin
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Product Details Cat. No.
Y01311
Purity
≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)
Endotoxin
≤10 EU/mg by the LAL method
Bioactivity
Measured by the ability of the immobilized protein to support the adhesion of NIH3T3 mouse embryonic fibroblast cells. The ED50
for this effect is 35.0-420 ng/mL.
Accession
P02751
Alternative Names
FN; Cold-insoluble globulin; CIG; FN; Fibronectin 1
Species
Human
Expression System
E.coli
Target Protein Sequence
PTDLRFTNIGPDTMRVTWAPPPSIDLTNFLVRYSPVKNEEDVAELSISPSDNAVVLTNLLPGTEYVVSVSSVYEQHESTPLRGRQKTGLDSPTGIDFSDITANSFTVHWIAPRATITGYRIRHHPEHFSGRPREDRVPHSRNSITLTNLTPGTEYVVSIVALNGREESPLLIGQQSTVSDVPRDLEVVAATPTSLLISWDAPAVTVRYYRITYGETGGNSPVQEFTVPGSKSTATISGLKPGVDYTITVYAVTGRGDSPASSKPISINYRTEIDKPSMAIPAPTDLKFTQVTPTSLSAQWTPPNVQLTGYRVRVTPKEKTGPMKEINLAPDSSSVVVSGLMVATKYEVSVYALKDTLTSRPAQGVVTTLENVSPPRRARVTDATETTITISWRTKTETITGFQVDAVPANGQTPIQRTIKPDVRSYTITGLQPGTDYKIYLYTLNDNARSSPVVIDASTAIDAPSNLRFLATTPNSLLVSWQPPRARITGYIIKYEKPGSPPREVVPRPRPGVTEATITGLEPGTEYTIYVIALKNNQKSEPLIGRKKTDELPQLVTLPHPNLHGPEILDVPST
Mol. Weight
62.6 kDa
Expression Region
Pro1270-Ser1546 & Ala1721-Thr2016
Tag Info
Tag free
Formulation
Lyophilized
Buffer
PBS,5% mannitol and 0.01% Tween 80, pH7.4
Size
10μg/vail 50μg/vail 1mg/vail
Lead Time
Products are in stock,but custom products may require further consultation
Reconstitution
It is recommended to redissolve in sterile deionized water.
Shipping
The product is shipped with blue ice
Storage & Stability
36 months at 2°C to 8°C in lyophilized state
6 months at -20°C to -80°C under sterile conditions after reconstitution
7-10 days at 2°C to 8°C under sterile conditions after reconstitution
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
SDS-PAGE
Recombinant Human Fibronectin (Cat. No. Y01311) SDS-PAGE
under reducing (R) & Non-reducing conditions. The gel was
stained with Y01311 SDS-PAGE.
Bioactivity
Recombinant Human Fibronectin (Cat. No. Y01311) stimulates
adhesion of NIH3T3 cells. The ED50 for this effect is
35.0-420 ng/mL. The bioactivity of Y01301 was higher than
other competing products.
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