Y01301 Recombinant Human Fibronectin

Cat#:Y01301

Name:Y01301 Recombinant Human Fibronectin

Species:Human

Specification:10μg/50μg/1mg

Purity:≥95%

Endotoxin: ≤10 EU/mg

Expression System:CHO

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Product Details

Cat. No.

Y01301

Purity

≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)

Endotoxin

≤10 EU/mg by the LAL method

Bioactivity

Measured by the ability of the immobilized protein to support the adhesion of NIH3T3 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 35.0-420 ng/mL.

Accession

P02751

Alternative Names

FN; Cold-insoluble globulin; CIG; FN; Fibronectin 1

Species

Human

Expression System

CHO

Target Protein Sequence

PTDLRFTNIGPDTMRVTWAPPPSIDLTNFLVRYSPVKNEEDVAELSISPSDNAVVLTNLLPGTEYVVSVSSVYEQHESTPLRGRQKTGLDSPTGIDFSDITANSFTVHWIAPRATITGYRIRHHPEHFSGRPREDRVPHSRNSITLTNLTPGTEYVVSIVALNGREESPLLIGQQSTVSDVPRDLEVVAATPTSLLISWDAPAVTVRYYRITYGETGGNSPVQEFTVPGSKSTATISGLKPGVDYTITVYAVTGRGDSPASSKPISINYRTEIDKPSMAIPAPTDLKFTQVTPTSLSAQWTPPNVQLTGYRVRVTPKEKTGPMKEINLAPDSSSVVVSGLMVATKYEVSVYALKDTLTSRPAQGVVTTLENVSPPRRARVTDATETTITISWRTKTETITGFQVDAVPANGQTPIQRTIKPDVRSYTITGLQPGTDYKIYLYTLNDNARSSPVVIDASTAIDAPSNLRFLATTPNSLLVSWQPPRARITGYIIKYEKPGSPPREVVPRPRPGVTEATITGLEPGTEYTIYVIALKNNQKSEPLIGRKKTDELPQLVTLPHPNLHGPEILDVPST

Mol. Weight

62.6 kDa

Expression Region

Pro1270-Ser1546&Ala1721-Thr2016

Tag Info

Tag free

Form

Lyophilized

Buffer

PBS,5% mannitol and 0.01% Tween 80, pH7.4

Size

10μg/vail 50μg/vail 1mg/vail

Lead Time

Products are in stock,but custom products may require further consultation

Reconstitution

It is recommended to redissolve in sterile deionized water.

Shipping

The product is shipped with blue ice

Storage & Stability

36 months at 2°C to 8°C in lyophilized state

6 months at -20°C to -80°C under sterile conditions after reconstitution

7-10 days at 2°C to 8°C under sterile conditions after reconstitution

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Data

SDS-PAGE

Y01301 Recombinant Human Fibronectin..jpg

Recombinant Human Fibronectin (Cat. No. Y01301) SDS-PAGE under reducing (R) & Non-reducing conditions. The gel was stained with Y01301 SDS-PAGE.

Bioactivity

Recombinant Human Fibronectin (Cat. No. Y01301) stimulates adhesion of B16-F1 cells. The ED₅₀ for this effect is 35.0-420 ng/mL. The Bioactivity of Y01301 was higher than other competing products.

SEC-HPLC