M-MLV Reverse Transcriptase

Cat#: EB0118

Specification: 50 μL

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Product Introduction

M-MLV Reverse Transcriptase is the fourth generation M-MLV reverse transcriptase obtained through gene modification and recombination technology. Compared with the previous M-MLV reverse transcriptase, this enzyme has improved tolerance to inhibitors that interfere with cDNA synthesis and can perform stable and specific cDNA synthesis on samples from different sources. At the same time, the reaction is faster, the incubation time is reduced from >50 min to 10 min, and the ability of continuous synthesis (final product 12.3 kb) is improved compared with the previous version.

Product specifications

Components	Specifications
Reverse Transcriptase (M-MLV Reverse Transcriptase	200 U/μL, 50 μL

Storage conditions

-80℃ storage, avoid repeated freezing and thawing.

Data

Enzyme activity experiment

Figure 1. Amplification sensitivity of different types of templates. The RT-RPA reaction system includes recombinase, recombinase auxiliary protein, single-stranded DNA binding protein, DNA polymerase, reverse transcriptase 4.0, exonuclease III, PRA buffer, PEG and trehalose, which are reacted in a qPCR instrument at 42°C for 25 minutes. (A) An RNA virus template; (B) A DNA plasmid template.

Enzyme stability experiment

Figure 2. Reverse transcriptase stability. Reverse transcriptase was lyophilized in storage solution (A) and placed at 4°C for 21 days (B). The enzyme stability was determined using the RT-RPA reaction system. Template: RNA virus, template amount: 10⁴ and 10³ copies/reaction.