Y04701 Recombinant Human VEGF165 Product Brochure

Cat#:Y04701

Name:Y04701 Recombinant Human VEGF165

Species:Human

Specification:10μg/50μg/1mg

Purity:≥95%

Endotoxin: ≤10 EU/mg

Expression System:CHO

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Product Details

Cat. No.

Y04701

Purity

≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)

Endotoxin

≤10 EU/mg by the LAL method

Bioactivity

Measured in a cell proliferation assay using HUVEC human umbilicveinendothelial cells. The ED₅₀ for this effect is 0.2-5 ng/mL

Accession

P15692

Alternative Names

Vascular Endothelial Growth Factor Isoform 165; VEGF165

Species

Human

Expression System

CHO

Target Protein Sequence

APMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQENPCGPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR

Mol. Weight

19.1kDa

Expression Region

Ala27-Arg191

Tag Info

Tag free

Formulation

Lyophilized

Buffer

PBS, 5% mannitol and 0.01% Tween 80, pH 7.4

Size

10μg/vail 50μg/vail 1mg/vail

Lead Time

Products are in stock, but custom products may require further consultation.

Reconstitution

It is recommended to redissolve in sterile deionized water.

Shipping

The product is shipped with blue ice

Storage & Stability

36 months at 2°C to 8°C in lyophilized state

6 months at -20°C to -80°C under sterile conditions after reconstitution

7-10 days at 2°C to 8°C under sterile conditions after reconstitution

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Data

SDS-PAGE

Recombinant Human VEGF165 (Cat. No. Y04701) SDS-PAGE under reducing (R) & Non-reducing conditions. The gel was stained with Y04701 SDS-PAGE.

Bioactivity

Recombinant Human VEGF165 (Cat. No. Y04701) stimulates proliferation of HUVEC human umbilicvein endothelial cells. The ED₅₀ for this effect is 0.2-10 ng/mL. Y04701 was higher than the other competing product