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H002D/H002L Recombinant Human Fibronectin

H002D/H002L Recombinant Human Fibronectin

Cat#:H002D/H002L

Species:Human

Size:1L

Purity:≥95%

Endotoxin: ≤10 EU/mg

Application Details
Cat. No.

H002D/H002L

Purity

≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)

Endotoxin

≤10 EU/mg by the LAL method

Concentration

1000ppm

Additive Amount

0.25-0.2%

Bioactivity

Measured by the ability of the immobilized protein to support the adhesion of NIH3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 35.0-420 ng/mL. 

Accession

P02751

Alternative Names

FN; Cold-insoluble globulin; CIG; FN; Fibronectin 1

Species

Human

Expression System

E coli

Target Protein Sequence

PTDLRFTNIGPDTMRVTWAPPPSIDLTNFLVRYSPVKNEEDVAELSISPSDNAVVLTNLLPGTEYVVSVSSVYEQHESTPLRGRQKTGLDSPTGIDFSDITANSFTVHWIAPRATITGYRIRHHPEHFSGRPREDRVPHSRNSITLTNLTPGTEYVVSIVALNGREESPLLIGQQSTVSDVPRDLEVVAATPTSLLISWDAPAVTVRYYRITYGETGGNSPVQEFTVPGSKSTATISGLKPGVDYTITVYAVTGRGDSPASSKPISINYRTEIDKPSMAIPAPTDLKFTQVTPTSLSAQWTPPNVQLTGYRVRVTPKEKTGPMKEINLAPDSSSVVVSGLMVATKYEVSVYALKDTLTSRPAQGVVTTLENVSPPRRARVTDATETTITISWRTKTETITGFQVDAVPANGQTPIQRTIKPDVRSYTITGLQPGTDYKIYLYTLNDNARSSPVVIDASTAIDAPSNLRFLATTPNSLLVSWQPPRARITGYIIKYEKPGSPPREVVPRPRPGVTEATITGLEPGTEYTIYVIALKNNQKSEPLIGRKKTDELPQLVTLPHPNLHGPEILDVPST

Mol. Weight

62.6 kDa

Expression Region

Pro1270-Ser1546&Ala1721-Thr2016

Tag Info

Tag free

Formulation

Lyophilized

Buffer
PBS,5% mannitol and 0.01% Tween 80, pH7.4
Size

1L

Lead Time

Products are in stock,but custom products may require further consultation

Reconstitution

It is recommended to redissolve in sterile deionized water.

Shipping

The product is shipped with blue ice

Storage & Stability

36 months at 2°C to 8°C in lyophilized state

6 months at -20°C to -80°C under sterile conditions after reconstitution

7-10 days at 2°C to 8°C under sterile conditions after reconstitution

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

INCI code

202667-11828-2704

Application

Fading red after sun, barrier repair, wound healing,etc.

Data
Cytotoxicity test results
微信截图_20241023114758.png
Figure 1 Schematic representation of cytotoxicity ofthe subject Recombinant Human Fibronectin Solution Pro+ against HaCaT
微信截图_20241023114811.png
Table 1 Cytotoxicity results of the test substance Recombinant Human Fibronectin Solution Pro+ against HaCaT Table
Cell Scratch Test Result

Figure 2 Effect of subject recombinant human fibronectin solution Pro+ on the healing of HaCaT Scratches

Table 2 Table of results of the healing rate ofHaCaT scratches by Recombinant Human Fibronectin Solution Pro+, the test substance
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东抗生物