H003D/H003L Recombinant Human Noggin

Cat#:H003D/H003L

Species:Human

Size:1L

Purity:≥95%

Endotoxin: ≤10 EU/mg

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Application Details
Data
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Application Details

Cat. No.

H003D/H003L

Purity

≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)

Endotoxin

≤10 EU/mg by the LAL method

Concentration

1000ppm

Bioactivity

Determined by its ability to inhibit 5.0 ng/ml of BMP-4 induced alkaline phosphatase production by ATDC-5 chondrogenic cells. The expected ED₅₀ for this effect is 2.0-3.0 ng/ml of Noggin.

Accession

Q13253

Alternative Names

Noggin; NOG

Species

Human

Expression System

CHO

Target Protein Sequence

QHYLHIRPAPSDNLPLVDLIEHPDPIFDPKEKDLNETLLRSLLGGHYDPGFMATSPPEDRPGGGGGAAGGAEDLAELDQLLRQRPSGAMPSEIKGLEFSEGLAQGKKQRLSKKLRRKLQMWLWSQTFCPVLYAWNDLGSRFWPRYVKVGSCFSKRSCSVPEGMVCKPSKSVHLTVLRWRCQRRGGQRCGWIPIQYPIISECKCSC

Mol. Weight

23 kDa

Expression Region

Gln28-Cys232

Tag Info

Tag free

Formulation

Lyophilized

Buffer

PBS,5% mannitol and 0.01% Tween 80, pH7.4

Size

Lead Time

Products are in stock,but custom products may require further consultation

Reconstitution

It is recommended to redissolve in sterile deionized water.

Shipping

The product is shipped with blue ice

Storage & Stability

36 months at 2°C to 8°C in lyophilized state

6 months at -20°C to -80°C under sterile conditions after reconstitution

7-10 days at 2°C to 8°C under sterile conditions after reconstitution

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Application

Hair growth

Data

SDS-PAGE

Recombinant Human Noggin (Cat. No. H003D/H003L) SDS-PAGE under reducing (R) & Non-reducing conditions. The gel was stained with H003D/H003L SDS-PAGE.

Bioactivity

Measured by its ability to inhibit 5.0 ng/mlof BMP-4 induced alkaline phosphatase production by ATDC-5 chondrocenic cells. The Bioactivity of Recombinant Human Noggin （Cat No.H003D/H003L） is equivalent to competitor.

Bioactivity