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H014D/H014L Recombinant Human HGF

Cat#:H014D/H014L

Species:Human

Size:1L

Purity:≥95%

Endotoxin: ≤10 EU/mg

Application Details
Cat. No.

H014D/H014L

Purity

≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)

Endotoxin

≤10 EU/mg by the LAL method

Concentration

1000ppm

Bioactivity

Measured by its ability to induce IL-11 secretion by Saos‑2 human osteosarcoma cells.  The ED50 for this effect is ≤2.5 ng/ml.

Accession

P14210

Alternative Names

Hepatocyte growth factor; HPTA; HGF; SF; Scatter factor; Hepatopoietin-A

Species

Human

Expression System

CHO

Target Protein Sequence

QRKRRNTIHEFKKSAKTTLIKIDPALKIKTKKVNTADQCANRCTRNKGLPFTCKAFVFDKARKQCLWFPFNSMSSGVKKEFGHEFDLYENKDYIRNCIIGKGRSYKGTVSITKSGIKCQPWSSMIPHEHSFLPSSYRGKDLQENYCRNPRGEEGGPWCFTSNPEVRYEVCDIPQCSEVECMTCNGESYRGLMDHTESGKICQRWDHQTPHRHKFLPERYPDKGFDDNYCRNPDGQPRPWCYTLDPHTRWEYCAIKTCADNTMNDTDVPLETTECIQGQGEGYRGTVNTIWNGIPCQRWDSQYPHEHDMTPENFKCKDLRENYCRNPDGSESPWCFTTDPNIRVGYCSQIPNCDMSHGQDCYRGNGKNYMG/VAIL NLSQTRSGLTCSMWDKNMEDLHRHIFWEPDASKLNENYCRNPDDDAHGPWCYTGNPLIPWDYCPISRCEGDTTPTIVNLDHPVISCAKTKQLR&VVNGIPTRTNIGWMVSLRYRNKHICGGSLIKESWVLTARQCFPSRDLKDYEAWLGIHDVHGRGDEKCKQVLNVSQLVYGPEGSDLVLMKLARPAVLDDFVSTIDLPNYGCTIPEKTSCSVYGWGYTGLINYDGLLRVAHLYIMG/VAIL NEKCSQHHRGKVTLNESEICAGAEKIGSGPCEGDYGGPLVCEQHKMRMVLGVIVPGRGCAIPNRPGIFVRVAYYAKWIHKIILTYKVPQS

Mol. Weight

53.7 kDa ( α chain), 26 kDa ( β chain)

Expression Region

α chain:Gln 32 -Arg 494 & β chain:Val 495-Ser 728

Tag Info

Tag free

Formulation

Lyophilized

Buffer

PBS,5% mannitol and 0.01% Tween 80, pH7.4

Size

1L

Lead Time

Products are in stock,but custom products may require further consultation

Reconstitution

It is recommended to redissolve in sterile deionized water.

Shipping

The product is shipped with blue ice

Storage & Stability

36 months at 2°C to 8°C in lyophilized state

6 months at -20°C to -80°C under sterile conditions after reconstitution

7-10 days at 2°C to 8°C under sterile conditions after reconstitution

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Application

Hair growth, Wound healing, etc.

Data
Bioactivity

HGF_00.jpg

Measured by its ability to induce IL-11 secretion by Saos-2 human osteosarcoma cells.  The ED50 for this effect is ≤2.5 ng/ml.  The Bioactivity of H014D/H014L was higher than the other competing product.

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East Mab Bio

东抗生物