Cat#:Y04603
Name:Y04603 Recombinant Human Wnt-3a(Fc Tag）
Species:Human
Specification:10μg/50μg/1mg
Purity:≥95%
Endotoxin: ≤10 EU/mg
Expression System:CHO
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Y04603 Recombinant Human Wnt-3a(Fc Tag）
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Product Details Cat. No.
Y04603
Purity
≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)
Endotoxin
≤100 EU/mg by the LAL method
Bioactivity
Measured by its ability to induce Topflash reporterBioactivity in CHO-K1 cells. The ED50 for this effect is ＜30ng/ml.
Accession
NP_149122
Alternative Names
MG/VAIL C119418; MG/VAIL C119419; MG/VAIL C119420; protein Wnt-3a; wingless-type MMTV integration site family, member 3A; Wnt3a; Wnt-3a
Species
Human
Expression System
CHO
Target Protein Sequence
SYPIWWSLAVGPQYSSLGSQPILCASIPGLVPKQLRFCRNYVEIMPSVAEGIKIGIQECQHQFRGRRWNCTTVHDSLAIFGPVLDKATRESAFVHAIASAGVAFAVTRSCAEGTAAICGCSSRHQGSPGKGWKWGGCSEDIEFGGMVSREFADARENRPDARSAMNRHNNEAGRQAIASHMHLKCKCHGLSGSCEVKTCWWSQPDFRAIGDFLKDKYDSASEMVVEKHRESRGWVETLRPRYTYFKVPTERDLVYYEASPNFCEPNPETGSFGTRDRTCNVSSHGIDGCDLLCCGRGHNARAERRREKCRCVFHWCCYVSCQECTRVYDVHTCK
Mol. Weight
58.2 kDa
Expression Region
Ser19-Lys352 with a C-terminal linker GGGGGS and Human lgG1 (Asp104-Lys330)
Tag Info
Fc Tag
Formulation
Lyophilized
Buffer
Lyophilized from a 0.2 um filtered solution containing PBS, pH7.4
Size
10μg/vail 50μg/vail 1mg/vail
Lead Time
Products are in stock,but custom products may require further consultation
Reconstitution
It is recommended to redissolve in sterile deionized water.
Shipping
The product is shipped with blue ice
Storage & Stability
36 months at 2°C to 8°C in lyophilized state
6 months at -20°C to -80°C under sterile conditions after reconstitution
7-10 days at 2°C to 8°C under sterile conditions after reconstitution
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
SDS-PAGE
Recombinant Human Wnt-3a(Fc Tag) (Cat. No. Y 04603) SDS-PAGE under reducing (R) & Non-reducing conditions. The gel was stained with Y 04603 SDS-PAGE.
Bioactivity
Measured by its ability to induce Topflash reporter bioactivity in CHO cells in the presence of 5ng/ml BMP2. The ED50 for this effect is ＜30ng/ml. TheBioactivity of Recombinant Human Wnt-3a(Fc Tag）(cat no Y04603) was higher than other competing products.
Bioactivity
Highly consistent bioactivity between different production batches of Recombinant Human Wnt-3a(Fc Tag) (Cat. No. Y04063).
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